Modern Applications of DNA Amplification Techniques is popular PDF and ePub book, written by Dirk Lassner in 2013-11-11, it is a fantastic choice for those who relish reading online the Science genre. Let's immerse ourselves in this engaging Science book by exploring the summary and details provided below. Remember, Modern Applications of DNA Amplification Techniques can be Read Online from any device for your convenience.
Modern Applications of DNA Amplification Techniques Book PDF Summary
In the ten years since the first publication on PCR (Saiki et al. , 1985), this in vitro method of nucleic acid replication and modification has grown to rival in popularity traditional microbiological, genetical und technical procedures for cloning, sequencing, gene detecting and related procedures. To date the PCR literature has emphasized six main areas of application: genetic mapping, detection of mutations, genetic polymorphism, transcriptional splicing and regulation, molecular virology and quantitative procedures. The overwhelming focus of quantification of DNA or RNA by PCR has been on human microbiology and oncological problems. The exquisite sensitivity of PCR gives this method the ability to detect extremely rare DNAs, mRNAs, mRNAs in small numbers of cells or in small amounts of tissue, and mRNAs expressed in mixed-cell populations. However, the exact and accurate quantification of specific nucleic acids in biological samples is in spite of numerous publications in that field still a general problem: during the peR process, an unknown initial number of target sequences are used as a template from which a large quantity of specific product can be obtained. Although the amount of product formed is easy to determine, it is difficult to deduce the initial copy number of the target molecule because the efficiency of the peR is largely unknown.
Detail Book of Modern Applications of DNA Amplification Techniques PDF
- Author : Dirk Lassner
- Release : 11 November 2013
- Publisher : Springer
- ISBN : 9781461553793
- Genre : Science
- Total Page : 143 pages
- Language : English
- PDF File Size : 20,9 Mb
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