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Genetic Library Construction and Screening Book PDF Summary

Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.

Detail Book of Genetic Library Construction and Screening PDF

Genetic Library Construction and Screening
  • Author : R.C. Bird
  • Release : 06 December 2012
  • Publisher : Springer Science & Business Media
  • ISBN : 9783642564086
  • Genre : Science
  • Total Page : 244 pages
  • Language : English
  • PDF File Size : 13,6 Mb

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