DNA Cloning A Hands on Approach is popular PDF and ePub book, written by Seok-Yong Choi in 2019-04-17, it is a fantastic choice for those who relish reading online the Medical genre. Let's immerse ourselves in this engaging Medical book by exploring the summary and details provided below. Remember, DNA Cloning A Hands on Approach can be Read Online from any device for your convenience.
DNA Cloning A Hands on Approach Book PDF Summary
This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.
Detail Book of DNA Cloning A Hands on Approach PDF
- Author : Seok-Yong Choi
- Release : 17 April 2019
- Publisher : Springer
- ISBN : 9789402416626
- Genre : Medical
- Total Page : 131 pages
- Language : English
- PDF File Size : 11,5 Mb
If you're still pondering over how to secure a PDF or EPUB version of the book DNA Cloning A Hands on Approach by Seok-Yong Choi, don't worry! All you have to do is click the 'Get Book' buttons below to kick off your Download or Read Online journey. Just a friendly reminder: we don't upload or host the files ourselves.