Cryotechniques in Biological Electron Microscopy is popular PDF and ePub book, written by Rudolf A. Steinbrecht in 2012-12-06, it is a fantastic choice for those who relish reading online the Science genre. Let's immerse ourselves in this engaging Science book by exploring the summary and details provided below. Remember, Cryotechniques in Biological Electron Microscopy can be Read Online from any device for your convenience.
Cryotechniques in Biological Electron Microscopy Book PDF Summary
To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.
Detail Book of Cryotechniques in Biological Electron Microscopy PDF
- Author : Rudolf A. Steinbrecht
- Release : 06 December 2012
- Publisher : Springer Science & Business Media
- ISBN : 9783642728150
- Genre : Science
- Total Page : 308 pages
- Language : English
- PDF File Size : 19,7 Mb
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