Advanced Photon Counting is popular PDF and ePub book, written by Peter Kapusta in 2015-04-23, it is a fantastic choice for those who relish reading online the Science genre. Let's immerse ourselves in this engaging Science book by exploring the summary and details provided below. Remember, Advanced Photon Counting can be Read Online from any device for your convenience.
Advanced Photon Counting Book PDF Summary
This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Detail Book of Advanced Photon Counting PDF
- Author : Peter Kapusta
- Release : 23 April 2015
- Publisher : Springer
- ISBN : 9783319156361
- Genre : Science
- Total Page : 371 pages
- Language : English
- PDF File Size : 14,6 Mb
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